Hotspot

ycf58

Genome · 68619-69083 · 465 bp

Back to Dasyclonium

Pi 0.186

MarkerSeek score 79.70

Primer available yes

Label ycf58

Feature Metadata

label_name: ycf58
feature_type: gene
region: Genome
length_bp: 465
variable_sites: 153
pi: 0.186
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf58_p1	TGCTTCAATCTCAGCATTTGG	TTCAGGTAGACGTGCAAAAA	42.9	40.0	57.1	55.5	1467-1493 bp	0.75	45.3
ycf58_p2	TGCTTCAATCTCAGCATTTGG	TTTCAGGTAGACGTGCAAAA	42.9	40.0	57.1	55.5	1468-1494 bp	0.75	45.3
ycf58_p3	TGCTTCAATCTCAGCATTTGG	TTTCAGGTAGACGTGCAAAAA	42.9	38.1	57.1	56.3	1468-1494 bp	0.75	43.6
ycf58_p4	TGCAGCAATTTCTAATGCCT	TTCAGGTAGACGTGCAAAAA	40.0	40.0	56.0	55.5	1800-1802 bp	0.5	26.9
ycf58_p5	TGCAGCAATTTCTAATGCCT	TTTCAGGTAGACGTGCAAAA	40.0	40.0	56.0	55.5	1801-1803 bp	0.5	26.9