Hotspot

ycf55

Genome · 15475-16422 · 948 bp

Back to Dasyclonium

Pi 0.129

MarkerSeek score 80.40

Primer available yes

Label ycf55

Feature Metadata

label_name: ycf55
feature_type: gene
region: Genome
length_bp: 948
variable_sites: 220
pi: 0.129
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf55_p1	TGTCCTACTGGAGCTTCTGT	AGTTTTGACTAGCAGCAGCA	50.0	45.0	57.7	57.7	1793 bp	0.25	45.4
ycf55_p2	TGTCCTACTGGAGCTTCTGT	TTGACTAGCAGCAGCAAAGT	50.0	45.0	57.7	57.7	1789 bp	0.25	45.4
ycf55_p3	TGTCCTACTGGAGCTTCTGT	CTGGACTTGGAGCTGCATTT	50.0	50.0	57.7	58.5	2294 bp	0.25	43.9
ycf55_p4	TGTCCTACTGGAGCTTCTGT	GGACTTGGAGCTGCATTTGT	50.0	50.0	57.7	58.7	2292 bp	0.25	41.5
ycf55_p5	TGTCCTACTGGAGCTTCTGT	TGGACTTGGAGCTGCATTTG	50.0	50.0	57.7	58.7	2293 bp	0.25	41.5