Hotspot

ycf4

Genome · 64869-65417 · 549 bp

Back to Dasyclonium

Pi 0.109

MarkerSeek score 82.80

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: Genome
length_bp: 549
variable_sites: 107
pi: 0.109
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	AAAATATCCGTGGGCCATGC	TCCAGCCAGATGCAATTCAA	50.0	45.0	59.0	57.8	1335-1337 bp	0.5	52.7
ycf4_p2	AAAATATCCGTGGGCCATGC	CCCGCATCATTAGCTTGGAA	50.0	50.0	59.0	58.3	1250-1251 bp	0.5	51.4
ycf4_p3	AAAATATCCGTGGGCCATGC	ATCCAGCCAGATGCAATTCA	50.0	45.0	59.0	57.6	1336-1338 bp	0.5	50.8
ycf4_p4	AAATATCCGTGGGCCATGC	TCCAGCCAGATGCAATTCAA	52.6	45.0	58.3	57.8	1334-1336 bp	0.5	50.2
ycf4_p5	AAAATATCCGTGGGCCATGC	ATCGAACCCGCATCATTAGC	50.0	50.0	59.0	58.5	1256-1257 bp	0.5	50.1