Hotspot

tilS

Genome · 33085-34065 · 981 bp

Back to Dasyclonium

Pi 0.154

MarkerSeek score 83.50

Primer available yes

Label tilS

Feature Metadata

label_name: tilS
feature_type: gene
region: Genome
length_bp: 981
variable_sites: 272
pi: 0.154
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tilS_p1	GGTGCACCTCTATGTCCTTG	GCGGATACCTGTTACACCTG	55.0	55.0	58.0	58.1	2250 bp	0.25	49.2
tilS_p2	GCACCTCTATGTCCTTGTCG	GCGGATACCTGTTACACCTG	55.0	55.0	58.1	58.1	2247 bp	0.25	48.9
tilS_p3	ATGGTGCACCTCTATGTCCT	GCGGATACCTGTTACACCTG	50.0	55.0	58.1	58.1	2252 bp	0.25	48.6
tilS_p4	GGTGCACCTCTATGTCCTTG	CTCGAACCCGGAACTAATCG	55.0	55.0	58.0	58.2	1640 bp	0.25	48.5
tilS_p5	GCACCTCTATGTCCTTGTCG	CTCGAACCCGGAACTAATCG	55.0	55.0	58.1	58.2	1637 bp	0.25	48.1