Hotspot

orf5

Genome · 65782-66873 · 1092 bp

Back to Dasyclonium

Pi 0.213

MarkerSeek score 84.40

Primer available yes

Label orf5

Feature Metadata

label_name: orf5
feature_type: gene
region: Genome
length_bp: 1092
variable_sites: 400
pi: 0.213
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf5_p1	TTCCAAGCTAATGATGCGGG	CGGACTCATGTGTGATTCGT	50.0	50.0	58.3	58.0	1875-2013 bp	0.75	79.4
orf5_p2	TTGAATTGCATCTGGCTGGA	CGGACTCATGTGTGATTCGT	45.0	50.0	57.8	58.0	1927-1928 bp	0.5	60.9
orf5_p3	TTGAATTGCATCTGGCTGGA	AGCGGACTCATGTGTGATTC	45.0	50.0	57.8	58.0	1929-1930 bp	0.5	60.8
orf5_p4	CCCGCTACCCGCTTTAATAA	CGGACTCATGTGTGATTCGT	50.0	50.0	57.8	58.0	1986 bp	0.25	48.0
orf5_p5	CCCGCTACCCGCTTTAATAA	AGCGGACTCATGTGTGATTC	50.0	50.0	57.8	58.0	1988 bp	0.25	47.9