Hotspot

moeB

Genome · 151216-152301 · 1086 bp

Back to Dasyclonium

Pi 0.169

MarkerSeek score 79.50

Primer available yes

Label moeB

Feature Metadata

label_name: moeB
feature_type: gene
region: Genome
length_bp: 1086
variable_sites: 325
pi: 0.169
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
moeB_p1	TGTTTGTTGCTATTTCTGCA	AGAACAGAAGCTGGAGGAGT	35.0	50.0	54.0	58.0	1936-1946 bp	0.5	30.1
moeB_p2	TGTTTGTTGCTATTTCTGCA	CTACAAGCTGTACCGCGAAT	35.0	50.0	54.0	58.1	2104-2114 bp	0.5	29.7
moeB_p3	TGTTTGTTGCTATTTCTGCA	ACCAACCTTATCCGCTGTTC	35.0	50.0	54.0	57.9	1856-1866 bp	0.5	29.5
moeB_p4	TGTTTGTTGCTATTTCTGCA	ATAAACACGCCCTGAAGGAC	35.0	50.0	54.0	57.9	1508 bp	0.5	29.0
moeB_p5	TGTTTGTTGCTATTTCTGCA	GGAAACCTACGTTCTGCCAA	35.0	50.0	54.0	58.1	1465 bp	0.5	29.0