Hotspot

dsbD

Genome · 23624-24361 · 738 bp

Back to Dasyclonium

Pi 0.15

MarkerSeek score 81.00

Primer available yes

Label dsbD

Feature Metadata

label_name: dsbD
feature_type: gene
region: Genome
length_bp: 738
variable_sites: 196
pi: 0.15
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dsbD_p1	GGTTCAAATCCTTCCTGGGG	TAAACTTCCAACGTCGTGCA	55.0	45.0	58.1	58.1	1223 bp	0.25	48.3
dsbD_p2	ATTTCACCACGTCCGCTTTA	TAAACTTCCAACGTCGTGCA	45.0	45.0	57.8	58.1	1416 bp	0.25	48.1
dsbD_p3	TTTCACCACGTCCGCTTTAT	TAAACTTCCAACGTCGTGCA	45.0	45.0	57.8	58.1	1415 bp	0.25	48.1
dsbD_p4	TAGCGTGTCTGCCAATTTCA	TAAACTTCCAACGTCGTGCA	45.0	45.0	57.8	58.1	1430 bp	0.25	48.1
dsbD_p5	TTCACCACGTCCGCTTTATC	TAAACTTCCAACGTCGTGCA	50.0	45.0	58.3	58.1	1414 bp	0.25	47.2