Hotspot

accB

Genome · 112163-112648 · 486 bp

Back to Dasyclonium

Pi 0.126

MarkerSeek score 82.20

Primer available yes

Label accB

Feature Metadata

label_name: accB
feature_type: gene
region: Genome
length_bp: 486
variable_sites: 110
pi: 0.126
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accB_p1	ACTCAGGACCATCAGGGAAA	AATCGTGAGCATCTGCATGT	50.0	45.0	58.0	58.0	1414 bp	0.25	49.4
accB_p2	ACTCAGGACCATCAGGGAAA	GAAAACCACCTCCAACGTCT	50.0	50.0	58.0	58.0	1645 bp	0.25	49.3
accB_p3	ACTCAGGACCATCAGGGAAA	CACCTTGAAAACCACCTCCA	50.0	50.0	58.0	57.9	1651 bp	0.25	49.1
accB_p4	ACTCAGGACCATCAGGGAAA	ACACCTTGAAAACCACCTCC	50.0	50.0	58.0	57.9	1652 bp	0.25	49.1
accB_p5	ACTCAGGACCATCAGGGAAA	TGCCATCGAGATTCTCCTCT	50.0	50.0	58.0	57.9	1245 bp	0.25	49.0