Hotspot

Orf4

Genome · 70676-71368 · 693 bp

Back to Dasyclonium

Pi 0.199

MarkerSeek score 78.80

Primer available yes

Label Orf4

Feature Metadata

label_name: Orf4
feature_type: gene
region: Genome
length_bp: 693
variable_sites: 241
pi: 0.199
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
Orf4_p1	CCTAATTTCGCCACCTACCC	TCAAAAACAGCATCAACGGC	55.0	45.0	58.0	57.9	1277-1285 bp	0.5	61.5
Orf4_p2	CCTAATTTCGCCACCTACCC	AATTCTAATGGGCAGCGTGT	55.0	45.0	58.0	57.9	1499-1507 bp	0.5	61.3
Orf4_p3	CCTAATTTCGCCACCTACCC	AAAACAGCATCAACGGCAAC	55.0	45.0	58.0	58.1	1274-1282 bp	0.5	61.3
Orf4_p4	CCTAATTTCGCCACCTACCC	CAAAAACAGCATCAACGGCA	55.0	45.0	58.0	58.1	1276-1284 bp	0.5	61.3
Orf4_p5	TATTAGCTCTGCACCCCACT	AATTCTAATGGGCAGCGTGT	50.0	45.0	58.1	57.9	1779-1787 bp	0.5	60.5