Hotspot

trnL-trnN

Genome · 23385-23511 · 127 bp

Back to Dasyclonium

Pi 0.253

MarkerSeek score 80.30

Primer available yes

Label trnL-trnN

Feature Metadata

label_name: trnL-trnN
feature_type: igs
region: Genome
length_bp: 127
variable_sites: 53
pi: 0.253
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-trnN_p1	TAGCGTGTCTGCCAATTTCA	CTACCACTGAGCTACTGAGGA	45.0	52.4	57.8	58.0	159-178 bp	1.0	82.5
trnL-trnN_p2	TAGCGTGTCTGCCAATTTCA	TCTACCACTGAGCTACTGAGG	45.0	52.4	57.8	58.0	160-179 bp	1.0	82.5
trnL-trnN_p3	TAGCGTGTCTGCCAATTTCA	ACCAATCGGTTAACAGCCG	45.0	52.6	57.8	58.2	184-203 bp	1.0	81.4
trnL-trnN_p4	TAGCGTGTCTGCCAATTTCA	TACCACTGAGCTACTGAGGA	45.0	50.0	57.8	56.8	158-177 bp	1.0	81.0
trnL-trnN_p5	GTGTCTGCCAATTTCACCAC	CTACCACTGAGCTACTGAGGA	50.0	52.4	57.6	58.0	155-174 bp	1.0	80.6