primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf37_p1	ycf37	1	CCACTTTTAGTTCTCATTCTTGCT	ATGCAGTAGGATGGGTACGT	24	20	37.5	50.0	57.4	58.2	0.0	0.0	4.776674	6700	7212	1201-1201	0.500000	23.9
ycf37_p2	ycf37	2	TGCATTCATAATTGTTTGTCCACT	ATGCAGTAGGATGGGTACGT	24	20	33.3	50.0	57.7	58.2	0.0	0.0	4.554194	6700	7212	1220-1220	0.250000	13.6
ycf37_p3	ycf37	3	TGCATTCATAATTGTTTGTCCACT	TGTGATGCAGTAGGATGGGT	24	20	33.3	50.0	57.7	58.4	0.0	0.0	4.760160	6700	7212	1224-1224	0.250000	11.9
ycf37_p4	ycf37	4	TCCACTTTTAGTTCTCATTCTTGC	ATGCAGTAGGATGGGTACGT	24	20	37.5	50.0	57.4	58.2	0.0	0.0	4.770730	6700	7212	1202-1202	0.250000	11.8
ycf37_p5	ycf37	5	TGCATTCATAATTGTTTGTCCACT	TGCAGTAGGATGGGTACGTA	24	20	33.3	50.0	57.7	57.6	0.0	0.0	4.784233	6700	7212	1219-1219	0.250000	11.7
ycf55_p1	ycf55	1	TGTCCTACTGGAGCTTCTGT	AGTTTTGACTAGCAGCAGCA	20	20	50.0	45.0	57.7	57.7	9.7	3.2	0.571623	15475	16422	1793-1793	0.250000	45.4
ycf55_p2	ycf55	2	TGTCCTACTGGAGCTTCTGT	TTGACTAGCAGCAGCAAAGT	20	20	50.0	45.0	57.7	57.7	9.7	3.2	0.571623	15475	16422	1789-1789	0.250000	45.4
ycf55_p3	ycf55	3	TGTCCTACTGGAGCTTCTGT	CTGGACTTGGAGCTGCATTT	20	20	50.0	50.0	57.7	58.5	9.7	9.4	0.768287	15475	16422	2294-2294	0.250000	43.9
ycf55_p4	ycf55	4	TGTCCTACTGGAGCTTCTGT	GGACTTGGAGCTGCATTTGT	20	20	50.0	50.0	57.7	58.7	9.7	9.4	1.059528	15475	16422	2292-2292	0.250000	41.5
ycf55_p5	ycf55	5	TGTCCTACTGGAGCTTCTGT	TGGACTTGGAGCTGCATTTG	20	20	50.0	50.0	57.7	58.7	9.7	9.4	1.060451	15475	16422	2293-2293	0.250000	41.5
trnL-trnN_p1	trnL-trnN	1	TAGCGTGTCTGCCAATTTCA	CTACCACTGAGCTACTGAGGA	20	21	45.0	52.4	57.8	58.0	0.0	11.2	1.183505	23385	23511	159-178	1.000000	82.5
trnL-trnN_p2	trnL-trnN	2	TAGCGTGTCTGCCAATTTCA	TCTACCACTGAGCTACTGAGG	20	21	45.0	52.4	57.8	58.0	0.0	0.0	1.183505	23385	23511	160-179	1.000000	82.5
trnL-trnN_p3	trnL-trnN	3	TAGCGTGTCTGCCAATTTCA	ACCAATCGGTTAACAGCCG	20	19	45.0	52.6	57.8	58.2	0.0	8.1	1.328313	23385	23511	184-203	1.000000	81.4
trnL-trnN_p4	trnL-trnN	4	TAGCGTGTCTGCCAATTTCA	TACCACTGAGCTACTGAGGA	20	20	45.0	50.0	57.8	56.8	0.0	11.2	1.373578	23385	23511	158-177	1.000000	81.0
trnL-trnN_p5	trnL-trnN	5	GTGTCTGCCAATTTCACCAC	CTACCACTGAGCTACTGAGGA	20	21	50.0	52.4	57.6	58.0	0.0	11.2	1.428387	23385	23511	155-174	1.000000	80.6
dsbD_p1	dsbD	1	GGTTCAAATCCTTCCTGGGG	TAAACTTCCAACGTCGTGCA	20	20	55.0	45.0	58.1	58.1	0.0	0.0	0.210089	23624	24361	1223-1223	0.250000	48.3
dsbD_p2	dsbD	2	ATTTCACCACGTCCGCTTTA	TAAACTTCCAACGTCGTGCA	20	20	45.0	45.0	57.8	58.1	0.0	0.0	0.235573	23624	24361	1416-1416	0.250000	48.1
dsbD_p3	dsbD	3	TTTCACCACGTCCGCTTTAT	TAAACTTCCAACGTCGTGCA	20	20	45.0	45.0	57.8	58.1	0.0	0.0	0.235573	23624	24361	1415-1415	0.250000	48.1
dsbD_p4	dsbD	4	TAGCGTGTCTGCCAATTTCA	TAAACTTCCAACGTCGTGCA	20	20	45.0	45.0	57.8	58.1	0.0	0.0	0.240116	23624	24361	1430-1430	0.250000	48.1
dsbD_p5	dsbD	5	TTCACCACGTCCGCTTTATC	TAAACTTCCAACGTCGTGCA	20	20	50.0	45.0	58.3	58.1	0.0	0.0	0.345528	23624	24361	1414-1414	0.250000	47.2
tilS_p1	tilS	1	GGTGCACCTCTATGTCCTTG	GCGGATACCTGTTACACCTG	20	20	55.0	55.0	58.0	58.1	29.1	0.0	0.093906	33085	34065	2250-2250	0.250000	49.2
tilS_p2	tilS	2	GCACCTCTATGTCCTTGTCG	GCGGATACCTGTTACACCTG	20	20	55.0	55.0	58.1	58.1	0.0	0.0	0.136173	33085	34065	2247-2247	0.250000	48.9
tilS_p3	tilS	3	ATGGTGCACCTCTATGTCCT	GCGGATACCTGTTACACCTG	20	20	50.0	55.0	58.1	58.1	34.8	0.0	0.179018	33085	34065	2252-2252	0.250000	48.6
tilS_p4	tilS	4	GGTGCACCTCTATGTCCTTG	CTCGAACCCGGAACTAATCG	20	20	55.0	55.0	58.0	58.2	29.1	0.7	0.193455	33085	34065	1640-1640	0.250000	48.5
tilS_p5	tilS	5	GCACCTCTATGTCCTTGTCG	CTCGAACCCGGAACTAATCG	20	20	55.0	55.0	58.1	58.2	0.0	0.7	0.235722	33085	34065	1637-1637	0.250000	48.1
psaC-ycf22_p1	psaC-ycf22	1	AAATTGCTTCAGCGCCTAGA	CGAGGATCATCAAACCTCTGA	20	21	45.0	47.6	57.9	57.2	2.3	8.3	1.922327	46925	47899	1639-1643	0.500000	48.7
psaC-ycf22_p2	psaC-ycf22	2	AATTGCTTCAGCGCCTAGAA	CGAGGATCATCAAACCTCTGA	20	21	45.0	47.6	57.9	57.2	2.3	8.3	1.922327	46925	47899	1638-1642	0.500000	48.7
psaC-ycf22_p3	psaC-ycf22	3	AATTGCTTCAGCGCCTAGAA	TCGAGGATCATCAAACCTCTG	20	21	45.0	47.6	57.9	57.2	2.3	8.3	1.922327	46925	47899	1639-1643	0.500000	48.7
psaC-ycf22_p4	psaC-ycf22	4	CAGGTCAAATTGCTTCAGCG	CGAGGATCATCAAACCTCTGA	20	21	50.0	47.6	58.0	57.2	0.0	8.3	1.835328	46925	47899	1645-1645	0.250000	35.3
psaC-ycf22_p5	psaC-ycf22	5	CAGGTCAAATTGCTTCAGCG	TCGAGGATCATCAAACCTCTG	20	21	50.0	47.6	58.0	57.2	0.0	8.3	1.835328	46925	47899	1646-1646	0.250000	35.3
ssrA_p1	ssrA	1	ACTTTGGTGGTCGAAGGTTC	TCAGACATAATAACTTGGCGCT	20	22	50.0	40.9	58.0	57.9	0.0	7.6	2.173944	55012	55448	1314-1325	0.500000	44.9
ssrA_p2	ssrA	2	GACTTTGGTGGTCGAAGGTT	TCAGACATAATAACTTGGCGCT	20	22	50.0	40.9	58.0	57.9	0.0	7.6	2.173944	55012	55448	1315-1326	0.500000	44.9
ssrA_p3	ssrA	3	ACTTTGGTGGTCGAAGGTTC	ACAATTCTTTGGCGTTTCACT	20	21	50.0	38.1	58.0	56.9	0.0	0.0	2.112348	55012	55448	772-783	0.500000	44.3
ssrA_p4	ssrA	4	GACTTTGGTGGTCGAAGGTT	ACAATTCTTTGGCGTTTCACT	20	21	50.0	38.1	58.0	56.9	0.0	0.0	2.112348	55012	55448	773-784	0.500000	44.3
ssrA_p5	ssrA	5	CTTTGGTGGTCGAAGGTTCA	ACAATTCTTTGGCGTTTCACT	20	21	50.0	38.1	58.0	56.9	0.0	0.0	2.113727	55012	55448	771-782	0.500000	44.3
ycf4_p1	ycf4	1	AAAATATCCGTGGGCCATGC	TCCAGCCAGATGCAATTCAA	20	20	50.0	45.0	59.0	57.8	20.7	0.0	1.179224	64869	65417	1335-1337	0.500000	52.7
ycf4_p2	ycf4	2	AAAATATCCGTGGGCCATGC	CCCGCATCATTAGCTTGGAA	20	20	50.0	50.0	59.0	58.3	20.7	0.0	1.281004	64869	65417	1250-1251	0.500000	51.4
ycf4_p3	ycf4	3	AAAATATCCGTGGGCCATGC	ATCCAGCCAGATGCAATTCA	20	20	50.0	45.0	59.0	57.6	20.7	0.0	1.407552	64869	65417	1336-1338	0.500000	50.8
ycf4_p4	ycf4	4	AAATATCCGTGGGCCATGC	TCCAGCCAGATGCAATTCAA	19	20	52.6	45.0	58.3	57.8	20.7	0.0	1.486791	64869	65417	1334-1336	0.500000	50.2
ycf4_p5	ycf4	5	AAAATATCCGTGGGCCATGC	ATCGAACCCGCATCATTAGC	20	20	50.0	50.0	59.0	58.5	20.7	0.0	1.447217	64869	65417	1256-1257	0.500000	50.1
orf5_p1	orf5	1	TTCCAAGCTAATGATGCGGG	CGGACTCATGTGTGATTCGT	20	20	50.0	50.0	58.3	58.0	0.0	4.9	0.324569	65782	66873	1875-2013	0.750000	79.4
orf5_p2	orf5	2	TTGAATTGCATCTGGCTGGA	CGGACTCATGTGTGATTCGT	20	20	45.0	50.0	57.8	58.0	0.0	4.9	0.222789	65782	66873	1927-1928	0.500000	60.9
orf5_p3	orf5	3	TTGAATTGCATCTGGCTGGA	AGCGGACTCATGTGTGATTC	20	20	45.0	50.0	57.8	58.0	0.0	4.9	0.234816	65782	66873	1929-1930	0.500000	60.8
orf5_p4	orf5	4	CCCGCTACCCGCTTTAATAA	CGGACTCATGTGTGATTCGT	20	20	50.0	50.0	57.8	58.0	0.0	4.9	0.247310	65782	66873	1986-1986	0.250000	48.0
orf5_p5	orf5	5	CCCGCTACCCGCTTTAATAA	AGCGGACTCATGTGTGATTC	20	20	50.0	50.0	57.8	58.0	0.0	4.9	0.259338	65782	66873	1988-1988	0.250000	47.9
orf5-cemA_p1	orf5-cemA	1	TTTGCTTTCCATTCTGGCTT	TCCTTCAGCTGTTGCAACAT	20	20	40.0	45.0	56.1	58.0	0.0	31.2	1.908531	66874	67598	1072-1073	0.500000	46.2
orf5-cemA_p2	orf5-cemA	2	TTTTGCTTTCCATTCTGGCT	TCCTTCAGCTGTTGCAACAT	20	20	40.0	45.0	56.1	58.0	0.0	31.2	1.908531	66874	67598	1073-1074	0.500000	46.2
orf5-cemA_p3	orf5-cemA	3	TTTTGCTTTCCATTCTGGCTT	TCCTTCAGCTGTTGCAACAT	21	20	38.1	45.0	56.9	58.0	0.0	31.2	2.136779	66874	67598	1073-1074	0.500000	44.4
orf5-cemA_p4	orf5-cemA	4	TTTTTGCTTTCCATTCTGGCT	TCCTTCAGCTGTTGCAACAT	21	20	38.1	45.0	56.9	58.0	0.0	31.2	2.136779	66874	67598	1074-1075	0.500000	44.4
orf5-cemA_p5	orf5-cemA	5	GTTTTTGCTTTCCATTCTGGC	TCCTTCAGCTGTTGCAACAT	21	20	42.9	45.0	56.8	58.0	0.0	31.2	2.169863	66874	67598	1075-1076	0.500000	44.1
ycf58_p1	ycf58	1	TGCTTCAATCTCAGCATTTGG	TTCAGGTAGACGTGCAAAAA	21	20	42.9	40.0	57.1	55.5	0.0	0.0	4.330678	68619	69083	1467-1493	0.750000	45.3
ycf58_p2	ycf58	2	TGCTTCAATCTCAGCATTTGG	TTTCAGGTAGACGTGCAAAA	21	20	42.9	40.0	57.1	55.5	0.0	0.0	4.330678	68619	69083	1468-1494	0.750000	45.3
ycf58_p3	ycf58	3	TGCTTCAATCTCAGCATTTGG	TTTCAGGTAGACGTGCAAAAA	21	21	42.9	38.1	57.1	56.3	0.0	0.0	4.544330	68619	69083	1468-1494	0.750000	43.6
ycf58_p4	ycf58	4	TGCAGCAATTTCTAATGCCT	TTCAGGTAGACGTGCAAAAA	20	20	40.0	40.0	56.0	55.5	0.0	0.0	4.487784	68619	69083	1800-1802	0.500000	26.9
ycf58_p5	ycf58	5	TGCAGCAATTTCTAATGCCT	TTTCAGGTAGACGTGCAAAA	20	20	40.0	40.0	56.0	55.5	0.0	0.0	4.487784	68619	69083	1801-1803	0.500000	26.9
Orf4_p1	Orf4	1	CCTAATTTCGCCACCTACCC	TCAAAAACAGCATCAACGGC	20	20	55.0	45.0	58.0	57.9	0.0	0.0	0.169568	70676	71368	1277-1285	0.500000	61.5
Orf4_p2	Orf4	2	CCTAATTTCGCCACCTACCC	AATTCTAATGGGCAGCGTGT	20	20	55.0	45.0	58.0	57.9	0.0	0.0	0.165598	70676	71368	1499-1507	0.500000	61.3
Orf4_p3	Orf4	3	CCTAATTTCGCCACCTACCC	AAAACAGCATCAACGGCAAC	20	20	55.0	45.0	58.0	58.1	0.0	0.0	0.185169	70676	71368	1274-1282	0.500000	61.3
Orf4_p4	Orf4	4	CCTAATTTCGCCACCTACCC	CAAAAACAGCATCAACGGCA	20	20	55.0	45.0	58.0	58.1	0.0	0.0	0.186451	70676	71368	1276-1284	0.500000	61.3
Orf4_p5	Orf4	5	TATTAGCTCTGCACCCCACT	AATTCTAATGGGCAGCGTGT	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.249196	70676	71368	1779-1787	0.500000	60.5
accB_p1	accB	1	ACTCAGGACCATCAGGGAAA	AATCGTGAGCATCTGCATGT	20	20	50.0	45.0	58.0	58.0	0.0	4.8	0.078897	112163	112648	1414-1414	0.250000	49.4
accB_p2	accB	2	ACTCAGGACCATCAGGGAAA	GAAAACCACCTCCAACGTCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.081621	112163	112648	1645-1645	0.250000	49.3
accB_p3	accB	3	ACTCAGGACCATCAGGGAAA	CACCTTGAAAACCACCTCCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.110691	112163	112648	1651-1651	0.250000	49.1
accB_p4	accB	4	ACTCAGGACCATCAGGGAAA	ACACCTTGAAAACCACCTCC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.112158	112163	112648	1652-1652	0.250000	49.1
accB_p5	accB	5	ACTCAGGACCATCAGGGAAA	TGCCATCGAGATTCTCCTCT	20	20	50.0	50.0	58.0	57.9	0.0	8.1	0.123962	112163	112648	1245-1245	0.250000	49.0
dnaB_p1	dnaB	1	TCAGTCCACTGCTCTACCAA	TGCAGAAATAGCAACAAACA	20	20	50.0	35.0	58.0	54.0	1.5	0.0	4.000759	148730	150556	2325-2325	0.250000	18.0
dnaB_p2	dnaB	2	TCCACTGCTCTACCAACTGA	TGCAGAAATAGCAACAAACA	20	20	50.0	35.0	58.0	54.0	0.0	0.0	4.000759	148730	150556	2321-2321	0.250000	18.0
dnaB_p3	dnaB	3	TTCAGTCCACTGCTCTACCA	TGCAGAAATAGCAACAAACA	20	20	50.0	35.0	58.0	54.0	1.5	0.0	4.000759	148730	150556	2326-2326	0.250000	18.0
dnaB_p4	dnaB	4	ACTGAGCTATCCCGGCTAAT	TGCAGAAATAGCAACAAACA	20	20	50.0	35.0	58.0	54.0	0.0	0.0	4.007982	148730	150556	2306-2306	0.250000	17.9
dnaB_p5	dnaB	5	AGTCCACTGCTCTACCAACT	TGCAGAAATAGCAACAAACA	20	20	50.0	35.0	58.0	54.0	0.0	0.0	4.007982	148730	150556	2323-2323	0.250000	17.9
moeB_p1	moeB	1	TGTTTGTTGCTATTTCTGCA	AGAACAGAAGCTGGAGGAGT	20	20	35.0	50.0	54.0	58.0	0.0	0.0	4.013795	151216	152301	1936-1946	0.500000	30.1
moeB_p2	moeB	2	TGTTTGTTGCTATTTCTGCA	CTACAAGCTGTACCGCGAAT	20	20	35.0	50.0	54.0	58.1	0.0	8.2	4.068507	151216	152301	2104-2114	0.500000	29.7
moeB_p3	moeB	3	TGTTTGTTGCTATTTCTGCA	ACCAACCTTATCCGCTGTTC	20	20	35.0	50.0	54.0	57.9	0.0	0.0	4.100502	151216	152301	1856-1866	0.500000	29.5
moeB_p4	moeB	4	TGTTTGTTGCTATTTCTGCA	ATAAACACGCCCTGAAGGAC	20	20	35.0	50.0	54.0	57.9	0.0	0.0	4.100502	151216	152301	1508-1508	0.500000	29.0
moeB_p5	moeB	5	TGTTTGTTGCTATTTCTGCA	GGAAACCTACGTTCTGCCAA	20	20	35.0	50.0	54.0	58.1	0.0	0.0	4.104191	151216	152301	1465-1465	0.500000	29.0
rps1_p1	rps1	1	CGCGTATGATGGAGTCACAA	ATTTCTGTGGCACGATCCTC	20	20	50.0	50.0	58.1	58.0	0.0	13.8	0.108595	157333	158127	1268-1283	1.000000	88.1
rps1_p2	rps1	2	GGAGTCACAATCCATTGCCT	ATTTCTGTGGCACGATCCTC	20	20	50.0	50.0	57.9	58.0	0.0	13.8	0.164627	157333	158127	1258-1273	1.000000	87.6
rps1_p3	rps1	3	TTATTGAGGGCGAATGACGG	ATTTCTGTGGCACGATCCTC	20	20	50.0	50.0	58.1	58.0	0.0	13.8	0.081734	157333	158127	1298-1307	0.750000	77.9
rps1_p4	rps1	4	TTATTGAGGGCGAATGACGG	ACGTACCATTGCACCCTTAC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.155433	157333	158127	1336-1345	0.750000	77.3
rps1_p5	rps1	5	TTATTGAGGGCGAATGACGG	AATAGAGCCCGGACTTTCCT	20	20	50.0	50.0	58.1	58.1	0.0	0.7	0.170955	157333	158127	1237-1246	0.750000	77.2
syfB_p1	syfB	1	GAGGATCGTGCCACAGAAAT	CGAATCCTCCTACCCCAGAT	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.046743	158561	160549	2876-2889	1.000000	88.8
syfB_p2	syfB	2	AGGAAAGTCCGGGCTCTATT	CGAATCCTCCTACCCCAGAT	20	20	50.0	55.0	58.1	58.0	10.2	0.0	0.135964	158561	160549	2937-2950	1.000000	88.1
syfB_p3	syfB	3	GAGGATCGTGCCACAGAAAT	GGTGTAGCCAAGTGGTAAGG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.139263	158561	160549	2927-2940	1.000000	88.0
syfB_p4	syfB	4	GAGGATCGTGCCACAGAAAT	CTAACTCGATCTCGCGATGG	20	20	50.0	55.0	58.0	58.0	0.0	10.4	0.072750	158561	160549	2676-2677	0.750000	78.1
syfB_p5	syfB	5	GTAAGGGTGCAATGGTACGT	CGAATCCTCCTACCCCAGAT	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.120442	158561	160549	2846-2847	0.500000	61.2