Hotspot

ycf1

SSC · 122876-128587 · 5712 bp

Back to Dasiphora

Pi 0.0049

MarkerSeek score 41.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5712
variable_sites: 42
pi: 0.0049
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACTGCTTGGGTCAGTATCAA	TGATCGGTTAGCATATAAATCT	45.0	31.8	56.5	52.6	307 bp	1.0	41.6
ycf1_p2	ACTGCTTGGGTCAGTATCAA	ATGATCGGTTAGCATATAAATCT	45.0	30.4	56.5	53.2	308 bp	1.0	41.6
ycf1_p3	ACTGCTTGGGTCAGTATCAA	ACAATATAGATATGATCGGTTAGCA	45.0	32.0	56.5	55.6	319 bp	1.0	41.5
ycf1_p4	ACTGCTTGGGTCAGTATCAA	AACAATATAGATATGATCGGTTAGCA	45.0	30.8	56.5	56.2	320 bp	1.0	41.5
ycf1_p5	ACTGCTTGGGTCAGTATCAAT	ACAATATAGATATGATCGGTTAGCA	42.9	32.0	57.0	55.6	319 bp	1.0	41.5