Hotspot

ccsA-ndhD

SSC · 114135-114416 · 282 bp

Back to Dasiphora

Pi 0.0119

MarkerSeek score 62.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 282
variable_sites: 5
pi: 0.0119
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATTCTGCAATTGTGGCCTCA	CAGTTGACAAGGTCGAAGCT	45.0	50.0	58.1	58.1	440-445 bp	1.0	80.4
ccsA-ndhD_p2	ATTCTGCAATTGTGGCCTCA	TCAGTTGACAAGGTCGAAGC	45.0	50.0	58.1	58.1	441-446 bp	1.0	80.3
ccsA-ndhD_p3	TTCTGCAATTGTGGCCTCAG	CAGTTGACAAGGTCGAAGCT	50.0	50.0	58.7	58.1	439-444 bp	1.0	75.0
ccsA-ndhD_p4	TTCTGCAATTGTGGCCTCAG	TCAGTTGACAAGGTCGAAGC	50.0	50.0	58.7	58.1	440-445 bp	1.0	74.9
ccsA-ndhD_p5	ATTCTGCAATTGTGGCCTCA	CAGTTGACAAGGTCGAAGCTA	45.0	47.6	58.1	58.0	440-445 bp	1.0	73.2