Hotspot

rpl32-trnL-UAG

SSC · 112340-113002 · 663 bp

Back to Dasiphora

Pi 0.0131
MarkerSeek score 54.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
663
variable_sites
13
pi
0.0131
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGCAGCATTGAAAGCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 775-810 bp 1.0 80.0
rpl32-trnL-UAG_p2 GGGCAGCATTGAAAGCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 776-811 bp 1.0 80.0
rpl32-trnL-UAG_p3 GGGCAGCATTGAAAGCTTTT ATCCACACAAAAGAGCTGCA 45.0 45.0 57.8 58.0 1372-1407 bp 1.0 79.5
rpl32-trnL-UAG_p4 GGGCAGCATTGAAAGCTTTT TCCACACAAAAGAGCTGCAT 45.0 45.0 57.8 58.0 1371-1406 bp 1.0 79.5
rpl32-trnL-UAG_p5 GGGCAGCATTGAAAGCTTTT ATAACACTTGGCGCCGTTAT 45.0 45.0 57.8 58.0 1232-1267 bp 1.0 79.4