Hotspot

rpl36-rps8

LSC · 79376-79898 · 523 bp

Back to Dasiphora

Pi 0.00637

MarkerSeek score 66.70

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 523
variable_sites: 5
pi: 0.00637
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	TAATTCGTCCCCGTCTACGA	GAATCGGCGGGGAAATTTTG	50.0	50.0	58.0	58.1	604-671 bp	1.0	82.9
rpl36-rps8_p2	TAATTCGTCCCCGTCTACGA	GAATAGAAGGAATCGGCGGG	50.0	55.0	58.0	58.2	613-680 bp	1.0	81.8
rpl36-rps8_p3	TAATTCGTCCCCGTCTACGA	TCGAATAGAAGGAATCGGCG	50.0	50.0	58.0	57.6	615-682 bp	1.0	80.1
rpl36-rps8_p4	TAATTCGTCCCCGTCTACGA	CGAGGGATAATGACAGACCG	50.0	55.0	58.0	57.6	641-708 bp	1.0	79.9
rpl36-rps8_p5	TAATTCGTCCCCGTCTACGA	CGAATAGAAGGAATCGGCGG	50.0	55.0	58.0	58.6	614-681 bp	1.0	78.8