Hotspot

petA-psbJ

LSC · 62481-63446 · 966 bp

Back to Dasiphora

Pi 0.00415

MarkerSeek score 66.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 966
variable_sites: 6
pi: 0.00415
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AAGGTTCAGTTGGCCGAAAT	CCGGATTAGGTTCATCTCTGT	45.0	47.6	58.0	56.9	968-1029 bp	1.0	67.6
petA-psbJ_p2	TTGAGAAGGTTCAGTTGGCC	CCGGATTAGGTTCATCTCTGT	50.0	47.6	58.0	56.9	973-1034 bp	1.0	67.5
petA-psbJ_p3	ATCCATTACGTGTCCAAGGC	CCGGATTAGGTTCATCTCTGT	50.0	47.6	58.0	56.9	1057-1118 bp	1.0	67.3
petA-psbJ_p4	CATTACGTGTCCAAGGCCTT	CCGGATTAGGTTCATCTCTGT	50.0	47.6	58.2	56.9	1054-1115 bp	1.0	66.2
petA-psbJ_p5	AAGGTTCAGTTGGCCGAAAT	TCCGGATTAGGTTCATCTCTGT	45.0	45.5	58.0	58.4	969-1030 bp	1.0	65.6