Hotspot

rbcL-accD

LSC · 56621-57287 · 667 bp

Back to Dasiphora

Pi 0.012

MarkerSeek score 71.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 667
variable_sites: 12
pi: 0.012
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CGCTCGTGAGGGTAATGAAA	GCCTACGCCCGTATTCTAAC	50.0	55.0	58.0	58.2	814-846 bp	1.0	82.4
rbcL-accD_p2	CGCTCGTGAGGGTAATGAAA	GTTAGCCTACGCCCGTATTC	50.0	55.0	58.0	58.2	818-850 bp	1.0	82.4
rbcL-accD_p3	CGCTCGTGAGGGTAATGAAA	AACAAGAGCGAACCAAACCA	50.0	45.0	58.0	58.2	785-817 bp	1.0	82.4
rbcL-accD_p4	CGCTCGTGAGGGTAATGAAA	CGAACCAAACCACCGTTTTT	50.0	45.0	58.0	57.7	777-809 bp	1.0	82.1
rbcL-accD_p5	CGCTCGTGAGGGTAATGAAA	AGCCTACGCCCGTATTCTAA	50.0	50.0	58.0	58.3	815-847 bp	1.0	81.7