Hotspot

ndhC-trnV-UAC

LSC · 50840-51436 · 597 bp

Back to Dasiphora

Pi 0.0214

MarkerSeek score 77.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 597
variable_sites: 16
pi: 0.0214
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	GCACGTTGGGTCTTTGAAAC	50.0	50.0	57.9	58.2	751-792 bp	1.0	83.2
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	687-728 bp	1.0	83.1
ndhC-trnV-UAC_p3	TTCTGGCCCTTTGCTAATCG	GCACGTTGGGTCTTTGAAAC	50.0	50.0	58.2	58.2	770-811 bp	1.0	82.3
ndhC-trnV-UAC_p4	TTCTGGCCCTTTGCTAATCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	706-747 bp	1.0	82.2
ndhC-trnV-UAC_p5	CCTTTGCTAATCGGGGCTAA	GCACGTTGGGTCTTTGAAAC	50.0	50.0	57.7	58.2	763-804 bp	1.0	81.6