Hotspot

trnF-GAA-ndhJ

LSC · 48395-49145 · 751 bp

Back to Dasiphora

Pi 0.00888

MarkerSeek score 56.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 751
variable_sites: 10
pi: 0.00888
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TGAAAGTTGGATAGGCTGGC	55.0	50.0	58.0	57.9	897-924 bp	1.0	80.1
trnF-GAA-ndhJ_p2	AATGAGGATGGTGCGTCATC	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.0	57.9	924-951 bp	1.0	79.8
trnF-GAA-ndhJ_p3	GAGGATGGTGCGTCATCAAT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.0	57.9	921-948 bp	1.0	79.8
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	861-888 bp	1.0	79.7
trnF-GAA-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	TGAAAGTTGGATAGGCTGGC	55.0	50.0	57.9	57.9	885-912 bp	1.0	79.0