Hotspot

petN-psbM

LSC · 27955-29201 · 1247 bp

Back to Dasiphora

Pi 0.00803

MarkerSeek score 64.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1247
variable_sites: 15
pi: 0.00803
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTAAATCTCGCTTGGGCTG	GTAAAAACAGTCAGCCAAGGC	50.0	47.6	58.0	58.0	1356-1377 bp	1.0	72.8
petN-psbM_p2	AGTAAATCTCGCTTGGGCTG	CGTAAAAACAGTCAGCCAAGG	50.0	47.6	58.0	57.7	1357-1378 bp	1.0	70.7
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	GTAAAAACAGTCAGCCAAGGC	50.0	47.6	57.6	58.0	1296-1317 bp	1.0	70.2
petN-psbM_p4	AGTAAATCTCGCTTGGGCTG	TAAAAACAGTCAGCCAAGGC	50.0	45.0	58.0	56.5	1355-1376 bp	1.0	69.2
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	CGTAAAAACAGTCAGCCAAGG	50.0	47.6	57.6	57.7	1297-1318 bp	1.0	68.2