Hotspot

trnG-UCC-trnR-UCU

LSC · 9361-9575 · 215 bp

Back to Dasiphora

Pi 0.0326

MarkerSeek score 65.10

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 215
variable_sites: 10
pi: 0.0326
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGGGAAATAAACTGCGTCCA	50.0	45.0	58.0	57.1	343-357 bp	1.0	76.0
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	AGGGAAATAAACTGCGTCCA	55.0	45.0	58.1	57.1	346-360 bp	1.0	75.7
trnG-UCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGGGAAATAAACTGCGTCCAA	50.0	42.9	58.0	57.9	343-357 bp	1.0	73.8
trnG-UCC-trnR-UCU_p4	CCCTAGCCTTCCAAGCTAAC	AGGGAAATAAACTGCGTCCA	55.0	45.0	57.7	57.1	347-361 bp	1.0	73.5
trnG-UCC-trnR-UCU_p5	CCTAGCCTTCCAAGCTAACG	AGGGAAATAAACTGCGTCCAA	55.0	42.9	58.1	57.9	346-360 bp	1.0	73.5