Hotspot

rpoC1

LSC · 21670-24498 · 2829 bp

Back to Daphniphyllum

Pi 0.00177

MarkerSeek score 55.60

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2829
variable_sites: 10
pi: 0.00177
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	AGAAGACCGAATTCACACCG	TTGCTCGTTCGAGAACTACG	50.0	50.0	57.9	58.0	2956-2980 bp	1.0	80.0
rpoC1_p2	AGAAGACCGAATTCACACCG	ACGATCTTTGGCTCTGGAAC	50.0	50.0	57.9	57.9	2939-2963 bp	1.0	79.5
rpoC1_p3	GAAGACCGAATTCACACCGT	TTGCTCGTTCGAGAACTACG	50.0	50.0	58.2	58.0	2955-2979 bp	1.0	78.9
rpoC1_p4	GAAGACCGAATTCACACCGT	ACGATCTTTGGCTCTGGAAC	50.0	50.0	58.2	57.9	2938-2962 bp	1.0	78.5
rpoC1_p5	AGAAGACCGAATTCACACCG	CGATCTTTGGCTCTGGAACT	50.0	50.0	57.9	57.6	2938-2962 bp	1.0	77.1