Hotspot

ccsA

SSC · 119579-120544 · 966 bp

Back to Daphniphyllum

Pi 0.00342

MarkerSeek score 62.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 6
pi: 0.00342
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGCGGCATGAGATCTTCTAA	GTTTGTATTCGTCAGGCCCT	50.0	50.0	57.5	57.9	1090-1099 bp	1.0	76.0
ccsA_p2	GGCGGCATGAGATCTTCTAA	ACGCCGTCCTATGTTTTGTT	50.0	45.0	57.5	58.1	1107-1116 bp	1.0	75.9
ccsA_p3	GGCGGCATGAGATCTTCTAA	ATTCGTCAGGCCCTTTCTTC	50.0	50.0	57.5	57.9	1084-1093 bp	1.0	75.9
ccsA_p4	TGGCGGCATGAGATCTTCTA	GTTTGTATTCGTCAGGCCCT	50.0	50.0	58.6	57.9	1091-1100 bp	1.0	75.5
ccsA_p5	TGGCGGCATGAGATCTTCTA	ACGCCGTCCTATGTTTTGTT	50.0	45.0	58.6	58.1	1108-1117 bp	1.0	75.5