Hotspot

ndhF-rpl32

SSC · 116905-118028 · 1124 bp

Back to Daphniphyllum

Pi 0.00854

MarkerSeek score 52.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1124
variable_sites: 20
pi: 0.00854
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCGTCGGAAAAAGGAGAAGT	AAAAGCTTTTAACGCTGCCC	50.0	45.0	57.8	57.9	1312-1342 bp	1.0	79.2
ndhF-rpl32_p2	TTTGTTGCCGTCGGAAAAAG	AAAAGCTTTTAACGCTGCCC	45.0	45.0	57.8	57.9	1319-1349 bp	1.0	79.0
ndhF-rpl32_p3	CTGGAAGTGGAACGAAAGGT	AAAAGCTTTTAACGCTGCCC	50.0	45.0	57.7	57.9	1270-1300 bp	1.0	78.6
ndhF-rpl32_p4	CCGTCGGAAAAAGGAGAAGT	GCCCAACATCCCTTCCTTTT	50.0	50.0	57.8	58.4	1296-1326 bp	1.0	77.6
ndhF-rpl32_p5	TTTGTTGCCGTCGGAAAAAG	GCCCAACATCCCTTCCTTTT	45.0	50.0	57.8	58.4	1303-1333 bp	1.0	77.4