Hotspot

ycf1-ndhF

SSC · 114619-115431 · 813 bp

Back to Daphniphyllum

Pi 0.0139

MarkerSeek score 71.50

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 813
variable_sites: 19
pi: 0.0139
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ACGTCTTCTGGTTTGAAAAACC	TGGGGTAAAGAAGGACCAAA	40.9	45.0	57.6	56.2	887-908 bp	1.0	51.1
ycf1-ndhF_p2	ACGTCTTCTGGTTTGAAAAACC	TGGGGTAAAGAAGGACCAAAA	40.9	42.9	57.6	57.0	887-908 bp	1.0	49.4
ycf1-ndhF_p3	ACGTCTTCTGGTTTGAAAAACC	ATGGGGTAAAGAAGGACCAA	40.9	45.0	57.6	56.0	888-909 bp	1.0	49.2
ycf1-ndhF_p4	ACGTCTTCTGGTTTGAAAAACC	TCTATGGGGTAAAGAAGGACCA	40.9	45.5	57.6	57.9	891-912 bp	1.0	48.5
ycf1-ndhF_p5	ACGTCTTCTGGTTTGAAAAACC	ATGGGGTAAAGAAGGACCAAA	40.9	42.9	57.6	56.8	888-909 bp	1.0	47.6