Hotspot

ycf4-cemA

LSC · 63897-64793 · 897 bp

Back to Daphniphyllum

Pi 0.00379

MarkerSeek score 55.50

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 897
variable_sites: 7
pi: 0.00379
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	GAGATCCACCAGGGCAAAAA	45.0	50.0	58.1	58.1	998-1007 bp	1.0	80.1
ycf4-cemA_p2	ACAAAAAGCTGCGGAATTGG	GAGATCCACCAGGGCAAAAA	45.0	50.0	57.8	58.1	1014-1023 bp	1.0	79.7
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	GAGAGAGAGATCCACCAGGG	45.0	60.0	58.1	58.3	1004-1013 bp	1.0	78.4
ycf4-cemA_p4	GCCATTCCCTTGACTCGTAC	GAGATCCACCAGGGCAAAAA	55.0	50.0	58.3	58.1	1064-1073 bp	1.0	78.0
ycf4-cemA_p5	ACAAAAAGCTGCGGAATTGG	GAGAGAGAGATCCACCAGGG	45.0	60.0	57.8	58.3	1020-1029 bp	1.0	77.9