Hotspot

rbcL-accD

LSC · 59931-60579 · 649 bp

Back to Daphniphyllum

Pi 0.00185

MarkerSeek score 60.70

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 649
variable_sites: 2
pi: 0.00185
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	CAACATCGAATTGAACCGCC	55.0	50.0	58.0	58.1	817-821 bp	1.0	79.4
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	TGAACCGCCGTTTTTCCATA	55.0	45.0	58.0	58.1	806-810 bp	1.0	79.4
rbcL-accD_p3	GAGGCTAGCAAATGGAGTCC	AATTGAACCGCCGTTTTTCC	55.0	45.0	58.0	57.9	809-813 bp	1.0	79.1
rbcL-accD_p4	GAGGCTAGCAAATGGAGTCC	AACCGCCGTTTTTCCATAGA	55.0	45.0	58.0	57.8	804-808 bp	1.0	78.8
rbcL-accD_p5	GAGGCTAGCAAATGGAGTCC	CGAATTGAACCGCCGTTTTT	55.0	45.0	58.0	58.2	811-815 bp	1.0	78.4