Hotspot

trnE-UUC-trnT-GGU

LSC · 33005-33831 · 827 bp

Back to Daphniphyllum

Pi 0.00822
MarkerSeek score 51.90
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
827
variable_sites
15
pi
0.00822
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 968-1061 bp 1.0 80.2
trnE-UUC-trnT-GGU_p2 TTTCGTAGTACCCTACCCCC TACCGCTGAGTTAAAAGGGC 55.0 50.0 57.8 57.9 933-1026 bp 1.0 79.5
trnE-UUC-trnT-GGU_p3 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 930-1023 bp 1.0 79.5
trnE-UUC-trnT-GGU_p4 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 909-1002 bp 1.0 79.4
trnE-UUC-trnT-GGU_p5 TTTCGTAGTACCCTACCCCC CCGATGACTTACGCCTTACC 55.0 55.0 57.8 58.2 965-1058 bp 1.0 79.1