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atpH-atpI

LSC · 14414-15436 · 1023 bp

Back to Daphniphyllum

Pi 0.00177

MarkerSeek score 55.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1023
variable_sites: 3
pi: 0.00177
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1090-1111 bp	1.0	77.7
atpH-atpI_p2	CGCAATCCCTTCTACAGCTT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	58.0	58.5	1178-1199 bp	1.0	77.6
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.9	58.5	1146-1167 bp	1.0	77.0
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	AGGCGAATCTATGGAGGGTC	45.0	55.0	58.0	58.7	1062-1083 bp	1.0	76.6
atpH-atpI_p5	CGCAATCCCTTCTACAGCTT	AGGCGAATCTATGGAGGGTC	50.0	55.0	58.0	58.7	1150-1171 bp	1.0	76.5