Hotspot

psbK-psbI

LSC · 8112-8501 · 390 bp

Back to Daphniphyllum

Pi 0.00923

MarkerSeek score 44.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 390
variable_sites: 7
pi: 0.00923
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TCTTCACGTCCAGGATTACG	50.0	50.0	57.9	57.1	526-534 bp	1.0	73.3
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	523-531 bp	1.0	71.3
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	524-532 bp	1.0	71.3
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	525-533 bp	1.0	71.3
psbK-psbI_p5	AAATTACCAGAGGCCTACGC	TCTTCACGTCCAGGATTACG	50.0	50.0	57.7	57.1	610-618 bp	1.0	71.0