Hotspot

trnH-GUG-psbA

LSC · 102-510 · 409 bp

Back to Daphniphyllum

Pi 0.0103
MarkerSeek score 58.60
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
409
variable_sites
8
pi
0.0103
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 55.0 55.0 58.1 57.9 455-476 bp 1.0 83.8
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 458-479 bp 1.0 83.7
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 469-490 bp 1.0 83.6
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 468-489 bp 1.0 83.6
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 470-491 bp 1.0 83.6