Hotspot

trnL-UAG-rpl32

SSC · 126075-129129 · 3055 bp

Back to Daphne

Pi 0.0223
MarkerSeek score 70.10
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
3055
variable_sites
333
pi
0.0223
unique_haplotype_count
19
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 ACACATTCCAACCAGTTCCC AGTACTATACTAGTATGTGAAAGACA 50.0 30.8 58.0 54.8 742-935 bp 0.947368 49.9
trnL-UAG-rpl32_p2 CACATTCCAACCAGTTCCCA AGTACTATACTAGTATGTGAAAGACA 50.0 30.8 58.0 54.8 741-934 bp 0.947368 49.9
trnL-UAG-rpl32_p3 ACACATTCCAACCAGTTCCC GTACTATACTAGTATGTGAAAGACA 50.0 32.0 58.0 53.5 741-934 bp 0.947368 49.9
trnL-UAG-rpl32_p4 CACATTCCAACCAGTTCCCA GTACTATACTAGTATGTGAAAGACA 50.0 32.0 58.0 53.5 740-933 bp 0.947368 49.9
trnL-UAG-rpl32_p5 GGTCGTGTGAACCAAAAACC AGTACTATACTAGTATGTGAAAGACA 50.0 30.8 57.8 54.8 777-970 bp 0.842105 45.7