Hotspot

rps19-rpl2

IRb · 85110-85167 · 58 bp

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Pi 0.0689
MarkerSeek score 76.20
Primer available yes
Label rps19-rpl2

Feature Metadata

label_name
rps19-rpl2
feature_type
igs
region
IRb
length_bp
58
variable_sites
12
pi
0.0689
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.97
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19-rpl2_p1 CCCGGGACCAAGTTCTTATT TTGATTCTTCGTCGCCGTAG 50.0 50.0 57.2 58.1 185-199 bp 1.0 84.5
rps19-rpl2_p2 CCCGGGACCAAGTTCTTATT ATTTGATTCTTCGTCGCCGT 50.0 45.0 57.2 58.3 187-201 bp 1.0 82.9
rps19-rpl2_p3 CCCGGGACCAAGTTCTTATT TTTGATTCTTCGTCGCCGTA 50.0 45.0 57.2 57.6 186-200 bp 1.0 82.4
rps19-rpl2_p4 CCCGGGACCAAGTTCTTATT TTGGGGTTATCCTGCACTTG 50.0 50.0 57.2 57.8 243-257 bp 1.0 81.1
rps19-rpl2_p5 CCCGGGACCAAGTTCTTATT GGGCTCCGATTGGTAGAAAA 50.0 50.0 57.2 57.6 277-291 bp 1.0 78.4