Hotspot

psbZ-trnG-GCC

LSC · 36973-37505 · 533 bp

Back to Daphne

Pi 0.0174
MarkerSeek score 71.50
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
533
variable_sites
49
pi
0.0174
unique_haplotype_count
18
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTGCTTCTCCTGATGGTTGG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 690-827 bp 1.0 86.4
psbZ-trnG-GCC_p2 GCTTCTCCTGATGGTTGGTT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 688-825 bp 1.0 86.4
psbZ-trnG-GCC_p3 CTCCTGATGGTTGGTTGAGT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.1 57.8 684-821 bp 1.0 81.2
psbZ-trnG-GCC_p4 TGCTTCTCCTGATGGTTGGT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.9 57.8 689-826 bp 1.0 80.6
psbZ-trnG-GCC_p5 TCTCCTGATGGTTGGTTGAG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 56.8 57.8 685-822 bp 1.0 78.8