Hotspot

petN-psbM

LSC · 29253-30159 · 907 bp

Back to Daphne

Pi 0.0176
MarkerSeek score 72.80
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
907
variable_sites
79
pi
0.0176
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCTTGCTTGGGCTGCTTTAA AAACAGTCAGCCAAGGCAAT 45.0 45.0 58.0 58.3 681-1219 bp 1.0 88.0
petN-psbM_p2 TCTTGCTTGGGCTGCTTTAA AACAGTCAGCCAAGGCAATT 45.0 45.0 58.0 58.3 680-1218 bp 1.0 88.0
petN-psbM_p3 TCTTGCTTGGGCTGCTTTAA ATTGCTACTGCGCTGTTGAT 45.0 45.0 58.0 58.3 739-1277 bp 1.0 87.9
petN-psbM_p4 TCTTGCTTGGGCTGCTTTAA TATTGCTACTGCGCTGTTGA 45.0 45.0 58.0 57.6 740-1278 bp 1.0 86.9
petN-psbM_p5 AGTAAGTCTTGCTTGGGCTG ATTGCTACTGCGCTGTTGAT 50.0 45.0 57.8 58.3 745-1283 bp 1.0 86.4