Hotspot

rpoB-trnC-GCA

LSC · 27752-28909 · 1158 bp

Back to Daphne

Pi 0.0136
MarkerSeek score 69.50
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1158
variable_sites
86
pi
0.0136
unique_haplotype_count
18
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CCATTTTCATCCCCGAGCAT CTGGGGTAAAGGGATTTGCA 50.0 50.0 58.3 57.8 1193-1242 bp 1.0 85.5
rpoB-trnC-GCA_p2 CCATTTTCATCCCCGAGCAT TGGGGTAAAGGGATTTGCAG 50.0 50.0 58.3 57.8 1192-1241 bp 1.0 85.5
rpoB-trnC-GCA_p3 CCATTTTCATCCCCGAGCAT ACTGGGGTAAAGGGATTTGC 50.0 50.0 58.3 57.8 1194-1243 bp 1.0 85.4
rpoB-trnC-GCA_p4 CCATTTTCATCCCCGAGCAT GGGGTAAAGGGATTTGCAGT 50.0 50.0 58.3 57.8 1191-1240 bp 1.0 85.4
rpoB-trnC-GCA_p5 ATTCCCCCATTTTCATCCCC TGGGGTAAAGGGATTTGCAG 50.0 50.0 57.5 57.8 1198-1247 bp 1.0 83.9