Hotspot

atpH-atpI

LSC · 14448-15299 · 852 bp

Back to Daphne

Pi 0.0201
MarkerSeek score 72.00
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
852
variable_sites
92
pi
0.0201
unique_haplotype_count
19
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT ATGGAGGGTCATCATTGA 45.0 44.4 58.0 52.2 897-1275 bp 1.0 50.6
atpH-atpI_p2 AGCGGCAGAAATCAATGGAT ATGGAGGGTCATCATTGA 45.0 44.4 57.9 52.2 889-1267 bp 1.0 50.6
atpH-atpI_p3 AAGCGGCAGAAATCAATGGA ATGGAGGGTCATCATTGA 45.0 44.4 58.2 52.2 890-1268 bp 1.0 50.6
atpH-atpI_p4 GCAAGTCCTACAGCTAACCC ATGGAGGGTCATCATTGA 55.0 44.4 58.0 52.2 924-1302 bp 1.0 50.4
atpH-atpI_p5 CAGGCCCAATAGAAGCAAGT ATGGAGGGTCATCATTGA 50.0 44.4 57.9 52.2 938-1316 bp 1.0 50.3