Hotspot

trnS-GCU-trnG-UCC

LSC · 8846-9602 · 757 bp

Back to Daphne

Pi 0.0233
MarkerSeek score 70.20
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
757
variable_sites
90
pi
0.0233
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 499-885 bp 1.0 63.7
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 498-884 bp 1.0 63.7
trnS-GCU-trnG-UCC_p3 CGCACAACGGATTAGCAATC ACGAATCACACTTTTACCACT 50.0 38.1 57.9 55.5 514-900 bp 1.0 63.0
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 502-888 bp 1.0 62.6
trnS-GCU-trnG-UCC_p5 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 501-887 bp 1.0 62.6