Hotspot

rps16-trnQ-UUG

LSC · 6229-7506 · 1278 bp

Back to Daphne

Pi 0.0205
MarkerSeek score 67.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1278
variable_sites
127
pi
0.0205
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.94
markerseek_score
67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA TTTGGTCCCGCTATTCGAAG 45.0 50.0 58.1 58.0 1267-1397 bp 1.0 90.5
rps16-trnQ-UUG_p2 CCACATCGCTTCAAACGAAG TTTGGTCCCGCTATTCGAAG 50.0 50.0 57.8 58.0 1247-1377 bp 1.0 89.9
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GTTTTGGTCCCGCTATTCGA 45.0 50.0 58.1 58.3 1269-1399 bp 1.0 88.5
rps16-trnQ-UUG_p4 ACCACATCGCTTCAAACGAA TTTGGTCCCGCTATTCGAAG 45.0 50.0 58.4 58.0 1248-1378 bp 1.0 88.4
rps16-trnQ-UUG_p5 TACCACATCGCTTCAAACGA TTTGGTCCCGCTATTCGAAG 45.0 50.0 57.6 58.0 1249-1379 bp 1.0 88.2