Hotspot

trnH-GUG-psbA

LSC · 121-451 · 331 bp

Back to Daphne

Pi 0.0387
MarkerSeek score 75.00
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
331
variable_sites
52
pi
0.0387
unique_haplotype_count
18
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CTGCCTTAATCCACTTGGCT TCCCTCTAGATCTAGCTGCT 50.0 50.0 57.9 56.3 393-433 bp 1.0 77.6
trnH-GUG-psbA_p2 ACTGCCTTAATCCACTTGGC TCCCTCTAGATCTAGCTGCT 50.0 50.0 58.2 56.3 394-434 bp 1.0 77.4
trnH-GUG-psbA_p3 CTGCCTTAATCCACTTGGCT TTCCCTCTAGATCTAGCTGCT 50.0 47.6 57.9 57.1 394-434 bp 1.0 75.8
trnH-GUG-psbA_p4 ACTGCCTTAATCCACTTGGC TTCCCTCTAGATCTAGCTGCT 50.0 47.6 58.2 57.1 395-435 bp 1.0 75.6
trnH-GUG-psbA_p5 AATCCACTTGGCTACATCCG TCCCTCTAGATCTAGCTGCT 50.0 50.0 57.7 56.3 386-426 bp 0.947368 73.9