Hotspot

trnL-UAA

LSC · 21752-21801 · 50 bp

Back to Danxiaorchis

Pi 0.04

MarkerSeek score 72.60

Primer available yes

Label trnL-UAA

Feature Metadata

label_name: trnL-UAA
feature_type: tRNA
region: LSC
length_bp: 50
variable_sites: 2
pi: 0.04
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA_p1	GACGAGAATAAAGAGAGAGTCCC	AGCGAGGAAGTAAAATGGGC	47.8	50.0	57.8	58.2	145 bp	1.0	53.7
trnL-UAA_p2	GACGAGAATAAAGAGAGAGTCCC	GCGAGGAAGTAAAATGGGCT	47.8	50.0	57.8	58.2	144 bp	1.0	53.7
trnL-UAA_p3	GGACGAGAATAAAGAGAGAGTCC	AGCGAGGAAGTAAAATGGGC	47.8	50.0	57.8	58.2	146 bp	1.0	53.7
trnL-UAA_p4	GGACGAGAATAAAGAGAGAGTCC	GCGAGGAAGTAAAATGGGCT	47.8	50.0	57.8	58.2	145 bp	1.0	53.7
trnL-UAA_p5	CGAGAATAAAGAGAGAGTCCCA	GCGAGGAAGTAAAATGGGCT	45.5	50.0	56.7	58.2	142 bp	1.0	53.1