Hotspot

trnG-GCC

LSC · 14978-15042 · 65 bp

Back to Danxiaorchis

Pi 0.0154

MarkerSeek score 61.30

Primer available yes

Label trnG-GCC

Feature Metadata

label_name: trnG-GCC
feature_type: tRNA
region: LSC
length_bp: 65
variable_sites: 1
pi: 0.0154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC_p1	ACGCATCAGGAACGAAGAAA	AGTCAACTATACTGACAGAATCT	45.0	34.8	57.8	54.3	125 bp	1.0	40.6
trnG-GCC_p2	ACGCATCAGGAACGAAGAAA	ACTACTGTATTCACTACTATGCA	45.0	34.8	57.8	54.4	164 bp	1.0	40.5
trnG-GCC_p3	TATGTGTGGACATATGCGGC	AGTCAACTATACTGACAGAATCT	50.0	34.8	58.1	54.3	147 bp	1.0	40.5
trnG-GCC_p4	GTACGCATCAGGAACGAAGA	ACTACTGTATTCACTACTATGCA	50.0	34.8	57.7	54.4	166 bp	1.0	40.5
trnG-GCC_p5	TATGTGTGGACATATGCGGC	ACTACTGTATTCACTACTATGCA	50.0	34.8	58.1	54.4	186 bp	1.0	40.4