Hotspot

rps16

LSC · 3844-4896 · 1053 bp

Back to Danxiaorchis

Pi 0.00382

MarkerSeek score 61.10

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1053
variable_sites: 4
pi: 0.00382
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TCATGATTCTTGTGTTCCCT	TGGTTCAGTAAATGACTATTCATGA	40.0	32.0	54.2	56.3	1183-1189 bp	1.0	40.7
rps16_p2	TCATGATTCTTGTGTTCCCTT	TGGTTCAGTAAATGACTATTCATGA	38.1	32.0	55.1	56.3	1183-1189 bp	1.0	40.7
rps16_p3	TTCATGATTCTTGTGTTCCCT	TGGTTCAGTAAATGACTATTCATGA	38.1	32.0	55.1	56.3	1184-1190 bp	1.0	40.7
rps16_p4	TCATGATTCTTGTGTTCCCT	TGTATTGGTTCAGTAAATGACT	40.0	31.8	54.2	53.1	1188-1194 bp	1.0	40.7
rps16_p5	TCATGATTCTTGTGTTCCCTTTC	TGGTTCAGTAAATGACTATTCATGA	39.1	32.0	57.0	56.3	1183-1189 bp	1.0	40.7