Hotspot

rrn4.5S-rrn5S

SSC · 64553-64764 · 212 bp

Back to Danxiaorchis

Pi 0.0142

MarkerSeek score 60.60

Primer available yes

Label rrn4.5S-rrn5S

Feature Metadata

label_name: rrn4.5S-rrn5S
feature_type: igs
region: SSC
length_bp: 212
variable_sites: 3
pi: 0.0142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5S-rrn5S_p1	GAGACGAGCCGTTTCTCATT	ACCAAGTTCGGGATGGATTG	50.0	50.0	58.0	57.9	356 bp	1.0	79.6
rrn4.5S-rrn5S_p2	GAGACGAGCCGTTTCTCATT	CACCAAGTTCGGGATGGATT	50.0	50.0	58.0	57.9	357 bp	1.0	79.6
rrn4.5S-rrn5S_p3	AAATAAGGTCACGGCGAGAC	ACCAAGTTCGGGATGGATTG	50.0	50.0	58.0	57.9	371 bp	1.0	79.6
rrn4.5S-rrn5S_p4	AAATAAGGTCACGGCGAGAC	CACCAAGTTCGGGATGGATT	50.0	50.0	58.0	57.9	372 bp	1.0	79.6
rrn4.5S-rrn5S_p5	GAGACGAGCCGTTTCTCATT	ACGCCTAGGACACCAGAATA	50.0	50.0	58.0	57.8	323 bp	1.0	79.5