Hotspot

rps3-rpl22

IRb · 43154-43196 · 43 bp

Back to Danxiaorchis

Pi 0.0233

MarkerSeek score 65.90

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: IRb
length_bp: 43
variable_sites: 1
pi: 0.0233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	CCGAGTCTGAAACCAAGTGG	TCGAGGGCGTAGTTATCTCA	55.0	50.0	58.5	57.7	157 bp	1.0	73.9
rps3-rpl22_p2	CGAGTCTGAAACCAAGTGGA	TCGAGGGCGTAGTTATCTCA	50.0	50.0	57.5	57.7	156 bp	1.0	73.6
rps3-rpl22_p3	TCCGAGTCTGAAACCAAGTG	TCGAGGGCGTAGTTATCTCA	50.0	50.0	57.5	57.7	158 bp	1.0	73.6
rps3-rpl22_p4	CCGAGTCTGAAACCAAGTGG	AAAGTTAAGACCCCGAGCTC	55.0	50.0	58.5	57.2	175 bp	1.0	70.4
rps3-rpl22_p5	CGAGTCTGAAACCAAGTGGA	AAAGTTAAGACCCCGAGCTC	50.0	50.0	57.5	57.2	174 bp	1.0	70.1