Hotspot

trnD-GUC-petN

LSC · 13447-14432 · 986 bp

Back to Danxiaorchis

Pi 0.00636
MarkerSeek score 61.10
Primer available yes
Label trnD-GUC-petN

Feature Metadata

label_name
trnD-GUC-petN
feature_type
igs
region
LSC
length_bp
986
variable_sites
6
pi
0.00636
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-petN_p1 AGTTCAATCGGTCAGAGCAC TAGTAAGTCTCGCTTGGGCT 50.0 50.0 57.9 58.2 1092-1135 bp 1.0 81.6
trnD-GUC-petN_p2 AGTTCAATCGGTCAGAGCAC TAGTATGGGGAAGGAGTGGG 50.0 55.0 57.9 57.5 1035-1078 bp 1.0 79.1
trnD-GUC-petN_p3 AGTTCAATCGGTCAGAGCAC ATAGTAAGTCTCGCTTGGGC 50.0 50.0 57.9 57.1 1093-1136 bp 1.0 75.7
trnD-GUC-petN_p4 GTTCAATCGGTCAGAGCACC TAGTAAGTCTCGCTTGGGCT 55.0 50.0 58.9 58.2 1091-1134 bp 1.0 74.9
trnD-GUC-petN_p5 AGTTCAATCGGTCAGAGCAC TCGCTTGGGCTGCTTTAAA 50.0 47.4 57.9 58.0 1083-1126 bp 1.0 74.6