Hotspot

trnC-GCA-trnE-UUC

LSC · 11296-12848 · 1553 bp

Back to Danxiaorchis

Pi 0.00677

MarkerSeek score 58.30

Primer available yes

Label trnC-GCA-trnE-UUC

Feature Metadata

label_name: trnC-GCA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 1553
variable_sites: 10
pi: 0.00677
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-trnE-UUC_p1	CCAGTTCGAATCTGGGTGTT	GTCGCCTCCTTGAAAGAGAG	50.0	55.0	57.8	58.0	1625-1690 bp	1.0	82.6
trnC-GCA-trnE-UUC_p2	CCAGTTCGAATCTGGGTGTT	TCCACAGCCAACTCACTTTC	50.0	50.0	57.8	58.0	1679-1744 bp	1.0	82.3
trnC-GCA-trnE-UUC_p3	CCAGTTCGAATCTGGGTGTT	TCGCCTCCTTGAAAGAGAGA	50.0	50.0	57.8	58.1	1624-1689 bp	1.0	82.1
trnC-GCA-trnE-UUC_p4	CCAGTTCGAATCTGGGTGTT	TGTCCTGAACCACTAGACGA	50.0	50.0	57.8	57.7	1604-1669 bp	1.0	80.5
trnC-GCA-trnE-UUC_p5	AGTTCGAATCTGGGTGTTGC	GTCGCCTCCTTGAAAGAGAG	50.0	55.0	58.5	58.0	1623-1688 bp	1.0	80.3