Hotspot

rps2-trnC-GCA

LSC · 8182-11224 · 3043 bp

Back to Danxiaorchis

Pi 0.00494

MarkerSeek score 58.50

Primer available yes

Label rps2-trnC-GCA

Feature Metadata

label_name: rps2-trnC-GCA
feature_type: igs
region: LSC
length_bp: 3043
variable_sites: 15
pi: 0.00494
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-trnC-GCA_p1	GAGCACTCGAGCAGTTTCTT	ACGTCAAGTAACACCAAGAGT	50.0	42.9	58.2	57.2	217 bp	1.0	65.3
rps2-trnC-GCA_p2	TGTTGAATCCGGTACCAACC	ACGTCAAGTAACACCAAGAGT	50.0	42.9	57.8	57.2	302 bp	1.0	65.0
rps2-trnC-GCA_p3	CCAACCGGTATAATTCCCCC	ACGTCAAGTAACACCAAGAGT	55.0	42.9	57.7	57.2	288 bp	1.0	64.3
rps2-trnC-GCA_p4	GTAGAGCACTCGAGCAGTTT	ACGTCAAGTAACACCAAGAGT	50.0	42.9	57.6	57.2	220 bp	1.0	64.1
rps2-trnC-GCA_p5	AGCACTCGAGCAGTTTCTTG	ACGTCAAGTAACACCAAGAGT	50.0	42.9	58.5	57.2	216 bp	1.0	63.0