Hotspot

ndhF

SSC · 103784-105997 · 2214 bp

Back to Danthoniopsis

Pi 0.00813

MarkerSeek score 53.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2214
variable_sites: 18
pi: 0.00813
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGATGAGCCTTGTTTTGAGGA	AGGTTTGGTGAATCGGAAACT	42.9	42.9	57.5	57.8	2334 bp	1.0	58.8
ndhF_p2	TGCATTTTGATGAGCCTTGT	AGGTTTGGTGAATCGGAAACT	40.0	42.9	56.2	57.8	2341 bp	1.0	56.5
ndhF_p3	TGCATTTTGATGAGCCTTGTT	AGGTTTGGTGAATCGGAAACT	38.1	42.9	57.0	57.8	2341 bp	1.0	54.6
ndhF_p4	TTGCATTTTGATGAGCCTTGT	AGGTTTGGTGAATCGGAAACT	38.1	42.9	57.0	57.8	2342 bp	1.0	54.6
ndhF_p5	TGATGAGCCTTGTTTTGAGGA	GGTTTGGTGAATCGGAAACT	42.9	45.0	57.5	56.2	2333 bp	1.0	54.5