Hotspot

ndhG-ndhI

SSC · 112220-112477 · 258 bp

Back to Danthoniopsis

Pi 0.0251
MarkerSeek score 59.00
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
258
variable_sites
6
pi
0.0251
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 AGACTACTCCTAGACCCCCT TCTTTGAATTCAAGAACGATTACT 55.0 29.2 57.7 54.8 354-373 bp 1.0 45.3
ndhG-ndhI_p2 AGACTACTCCTAGACCCCCT AGAAAAATCTTTGAATTCAAGAACGA 55.0 26.9 57.7 56.6 361-380 bp 1.0 45.3
ndhG-ndhI_p3 AGACTACTCCTAGACCCCCT AAATCTTTGAATTCAAGAACGA 55.0 27.3 57.7 52.6 357-376 bp 1.0 45.3
ndhG-ndhI_p4 AGACTACTCCTAGACCCCCT AAAATCTTTGAATTCAAGAACGA 55.0 26.1 57.7 53.5 358-377 bp 1.0 45.3
ndhG-ndhI_p5 AGACTACTCCTAGACCCCCT TCTTTGAATTCAAGAACGATTAC 55.0 30.4 57.7 53.4 354-373 bp 1.0 45.3